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Alterations in Nitric Oxide Synthase Isoforms in Acute Lower Limb Ischemia and Reperfusion

Department of Surgery, Royal Free Hospital, London, UK, jcstsui{at}hotmail.com

Daryll M. Baker, PhD

Department of Surgery, Royal Free Hospital, London, UK

Sidney G. Shaw, PhD

Department of Clinical Research, University of Bern, Switzerland

Michael R. Dashwood, PhD

Department of Surgery, Royal Free Hospital, London, UK, Department of Clinical Biochemistry, Royal Free Hospital, London, UK

Alterations in nitric oxide synthase (NOS) are implicated in ischemia and ischemia-reperfusion injury. Changes in the 3 NOS isoforms in human skeletal muscle subjected to acute ischemia and reperfusion were studied. Muscle biopsies were taken from patients undergoing total knee replacement. Distribution of the specific NOS isoforms within muscle sections was studied using immunohistochemistry. NOS mRNA levels were measured using real-time reverse transcription-polymerase chain reaction and protein levels studied using Western blotting. NOS activity was also assessed using the citrulline assay. All 3 NOS isoforms were found in muscle sections associated with muscle fibers and microvessels. In muscle subjected to acute ischemia and reperfusion, NOS I/neuronal NOS mRNA and protein were elevated during reperfusion. NOS III/endothelial NOS was also upregulated at the protein level during reperfusion. No changes in NOS II/inducible NOS expression or NOS activity occurred. In conclusion, alterations in NOS I and III (neuronal NOS and endothelial NOS) at different levels occurred after acute ischemia and reperfusion in human skeletal muscle; however, this did not result in increased NOS activity. In the development of therapeutic agents based on manipulation of the NO pathway, targeting the appropriate NOS isoenzymes may be important.

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Angiology, Vol. 58, No. 5, 586-592 (2007)
DOI: 10.1177/0003319707305466


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Tsui, J. C. S. Articles by Dashwood, M. R. Search for Related Content PubMed PubMed Citation Articles by Tsui, J. C. S. Articles by Dashwood, M. R. Social Bookmarking                         What's this?