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Effects of Antioxidants on Endothelial Function in Human Saphenous Vein in an Ex vivo Model

Department of Vascular and Endovascular Surgery, Belfast City Hospital, Belfast, Northern Ireland, aneessharif{at}yahoo.co.uk

Ulvi Bayraktutan, PhD

Department of Medicine, Queens University of Belfast, Belfast, Northern Ireland, Division of Stroke Medicine, Nottingham City Hospital, Nottingham United Kingdom

Nityanand Arya, FRCS

Department of Vascular and Endovascular Surgery, Belfast City Hospital, Belfast, Northern Ireland

Stephen A. Badger, MD

Department of Vascular and Endovascular Surgery, Belfast City Hospital, Belfast, Northern Ireland

Mark E. O'Donnell, MRCS

Department of Vascular and Endovascular Surgery, Belfast City Hospital, Belfast, Northern Ireland

Ian S. Young, MD

Division of Stroke Medicine, Nottingham City Hospital, Nottingham United Kingdom

Chee V. Soong, MD

Department of Vascular and Endovascular Surgery, Belfast City Hospital, Belfast, Northern Ireland

This ex vivo study is aimed at determining the beneficial effects of antioxidant agents on human saphenous vein endothelial function. Vein rings harvested during infrainguinal bypass surgery were assessed in an organ bath for endothelium-dependent relaxation, initially without and then with the addition of 10 µM manganese tetrakis benzoic acid porphyrin (MnTBAP), 0.01% N-acetylcysteine (NAC), 0.02% NAC, 10 µM vitamin C, and 100 µM vitamin C. Fifty-five vein rings from 22 patients were analyzed. MnTBAP improved the endothelium-dependent relaxation when compared with control (57.0% vs 37.8%, P < .01). Addition of 0.01% or 0.02% NAC did not improve the endothelium-dependent vasorelaxation (28.2% vs 18.6%, P = ns and 37.8% vs 29.8%, P = ns, respectively). Although 10-µM vitamin C failed to improve endothelial function (50.6% vs 37.2%, P = ns), 100-µM vitamin C significantly enhanced endothelium-dependent relaxation (66.5% vs 38.3%, P < .001). These results suggest that the addition of MnTBAP and high-dose vitamin C can improve the endothelial function of harvested saphenous vein segments in an ex vivo model.

Key Words: endothelial dysfunction • ischemia • MnTBAP • N-acetylcysteine • vein graft • vitamin C

This version was published on August 1, 2009

Angiology, Vol. 60, No. 4, 448-454 (2009)
DOI: 10.1177/0003319708321186


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