SAGE Journals Online
Advertisement
Sign In to gain access to subscriptions and/or personal tools.

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Advertisement

Sign In to gain access to subscriptions and/or personal tools.
Angiology
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Caimi, G.
Right arrow Articles by Lo Presti, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caimi, G.
Right arrow Articles by Lo Presti, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Leukocyte Flow Properties, Polymorphonuclear Membrane Fluidity, and Cytosolic Ca 2+ Content in Subjects with Vascular Atherosclerotic Disease with and Without Noninsulin-Dependent Diabetes Mellitus

Gregorio Caimi

Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo

Baldassare Canino

Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo

Filippo Ferrara

Cattedra di Angiologia, Università degli Studi di Palermo, Palermo, Italy

Maria Montana

Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo

Francesco Meli

Cattedra di Angiologia, Università degli Studi di Palermo, Palermo, Italy

Anna Catania

Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo

Rosalia Lo Presti

Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo

The aim of this research was the evaluation of white blood cell (WBC) filtration, reflecting WBC flow properties, polymorphonuclear cell membrane fluidity, and cytosolic Ca 2+ content in subjects with vascular atherosclerotic disease (VAD) and in VAD subjects with noninsulin-dependent diabetes mellitus (NIDDM), in good hemodynamic balance. The authors examined WBC filtration (unfractionated, mononuclear [MN], polymorphonu clear [PMN] cells), using the St. George Filtrometer and considering, respectively, the initial relative flow rate (IRFR) and the clogging rate (CR); the PMN membrane fluidity, employing the fluorescent probe TMA-DPH and calculating the fluorescence polarization degree; and the PMN cytosolic Ca2+ content, adopting the fluorescent probe Fura 2-AM and considering the ratio between the Fura 2-Ca2+ complex and the unchelated Fura 2 fluorescence intensity.

The obtained data showed that only the filtration parameters (IRFR, CR) of unfrac tionated WBCs discriminated normal subjects from VAD groups, whereas the filtration parameters of MN and PMN cells did not demonstrate any distinction. PMN membrane fluidity did not distinguish normal subjects from VAD groups, whereas PMN cytosolic Ca2+ content was significantly increased in VAD groups in comparison with normal subjects. No relationship was evident between WBC filtration and plasma metabolic para meters ; the correlations obtained between PMN filtration and other PMN parameters need further investigation.

Angiology, Vol. 47, No. 8, 757-763 (1996)
DOI: 10.1177/000331979604700802
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




Advertisement