This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gu, J. Articles by Cichon, R. Search for Related Content PubMed PubMed Citation Articles by Gu, J. Articles by Cichon, R. Social Bookmarking                   What's this?

Physical Manipulation of Subcutaneously Implanted Cardiac Muscle in Rat Increased Plasma Atrial Natriuretic Peptide Concentration

Deborah Research Institute, Browns Mills, NJ

Connie Daloisio

Deborah Research Institute, Browns Mills, NJ

Michael D'Andrea

Deborah Research Institute, Browns Mills, NJ

S. Muralidharan

Deborah Research Institute, Browns Mills, NJ

Cheng-Zhi Yu

Deborah Research Institute, Browns Mills, NJ

Romuald Cichon

Deborah Research Institute, Browns Mills, NJ

To examine the effects of physical stretch on cardiac muscle endocrine activ ity, the authors transplanted whole neonatal hearts subcutaneously into the back and the ears of the correspondent mother (n=9). Seven days later, physical manipulation was applied on the implanted heart by stretching the skin and the subcutaneous tissue encasing the implanted cardiac muscle, for a period of five to ten minutes. Such manipulation was repeated approximately every seven days postoperatively for a total of two to four times for each rat. The plasma atrial natriuretic peptide (ANP) levels were measured by radioimmunoassay prior to and immediately following manipulation. Postmanipulation plasma ANP levels were found to increase from the premanipulation levels. At two weeks postimplant, the average increase was 290% with the highest single-speci men increase being nearly twelvefold. The increases observed at two and three weeks following implantation had Signed Rank Test p values of 0.015 and 0.042 respectively. The viability of the implanted hearts was confirmed by cell culture. Light microscopic immunocytochemistry detected ANP immunoreactivity in the implanted cardiocytes. The elevated plasma ANP concentration induced by the manipulation appeared to be correlated with the functional status of the im planted cardiocytes.

In conclusion: (1) Subcutaneously transplanted neonatal myocardiocytes survived for at least three to four weeks while retaining the ability to produce ANP. (2) Physical manipulation of implanted heart induced ANP release. There fore, cardiac ANP production and release is indeed stimulated by physical stretching.

Angiology, Vol. 43, No. 10, 810-817 (1992)
DOI: 10.1177/000331979204301003


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?