This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gasser, R. Articles by Fleckenstein-Grün, G. Search for Related Content PubMed PubMed Citation Articles by Gasser, R. Articles by Fleckenstein-Grün, G. Social Bookmarking                         What's this?

Restriction by Nitrendipine of Excessive Concentration of Free Intracellular Calcium Ions in Ventricular Myocardium of Hypertensive Rats

University of Freiburg

Markus Frey

Study Group for Calcium Antagonism, Physiological Institute, University of Freiburg, Freiburg, West Germany

Young Kun Byon

Study Group for Calcium Antagonism, Physiological Institute, University of Freiburg, Freiburg, West Germany

Gisa Fleckenstein-Grün

Study Group for Calcium Antagonism, Physiological Institute, University of Freiburg, Freiburg, West Germany

In earlier investigations it has been found that high arterial blood pressure of salt-sensitive DAHL rats (DAHL-S) that received a diet con taining 8% NaCl could be normalized by chronic treatment with specific calcium antagonists, as for instance, nitrendipine or anipamil. Parallel measurements on heart and arterial vasculature with atomic absorption spectrometry revealed that there was a rise in absolute calcium content upon development of hypertension, whereas under the influence of ni trendipine, not only the blood pres sure and heart weight but also myocardial (and arterial) tissue cal cium content remained in the physio logic range.

In the present study, an additional determination of the free intracellu lar calcium ion concentration with Ca-sensitive microelectrodes was car ried out. For technical reasons, the measurements were restricted to iso lated resting rat papillary muscles (stretched to optimal length in a per fusion bath of 1.5 ml at 30°C in nor mal Tyrode solution). The impale ment of the electrodes was considered adequate when the heights of Ca po tential and membrane potential were constant for more than twenty min utes. In order to produce severe sys temic hypertension, a group of six-week-old DAHL-S rats was fed with an 8% NaCl diet over five weeks. Another group of DAHL-S rats received additionally 150 mg/kg nitrendipine twice a day orally. Salt- resistant DAHL-R rats, also fed with an 8% NaCl diet, served as controls.

The findings of this preliminary study are as follows (n = 5 in each group):

Angiology, Vol. 39, No. 3, 246-252 (1988)
DOI: 10.1177/000331978803900307


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?